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Item #:
194885416
DEPC-Treated Water, Diethylpyrocarbonate-Treated Ultrapure Water, More Than 99.99% Purity, Lab-Grade Purified, Sterilization, RNase & DNase Free,
Item #:
194885416
GBP 21
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DEPC treated ultrapure water is essential for any laboratory requiring the utmost purity and safety in RNA experiments.
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What Stands Out
High Purity
Achieves over 99.99% purity, ensuring optimal conditions for sensitive laboratory experiments and minimizing the risk of contamination.
RNase & DNase Free
Specifically treated to eliminate RNase and DNase, protecting nucleic acids from degradation during critical biological research.
Convenient Packaging
Available in 500ml bottles, providing ample supply for extended laboratory use while maintaining easy handling and storage.
Product Details
| Brand Name | CryoKing |
| Manufacturer | BIOLOGIX |
| Part Number | DEPC-500-1 |
| Unit Count | 16.91 Fluid Ounces |
| Included Components | DPBS Buffer |
| Brand | CryoKing |
| Item Weight | 1.1 lbs (500 grams) |
Who Should Buy?
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Molecular Biologists
Ideal for experiments requiring high purity, such as DNA/RNA extraction, minimizing contamination risks.
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Research Laboratories
Perfect for various scientific applications where uncontaminated water is essential for reliable results.
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Educational Institutions
Beneficial for teaching laboratories that conduct experiments in molecular biology or biochemistry.
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General Purpose Use
Not suitable for everyday applications like drinking or cooking where ultrapure water is unnecessary.
Product Description
DEPC-Treated Water, Diethylpyrocarbonate-Treated Ultrapure Water, More Than 99.99% Purity, Lab-Grade Purified, Sterilization, RNase & DNase Free, Proteinase Free, 500ml/Bottle
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Customer Questions & Answers
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Question:
What is DEPC-treated water?
Answer: DEPC-treated water is ultrapure water that has been treated with diethylpyrocarbonate to ensure the removal of RNases and DNases. This treatment process is crucial for molecular biology applications, as these enzymes can degrade RNA and DNA samples. By using DEPC-treated water, researchers minimize contamination risks, enhancing the integrity of their experiments. It's particularly beneficial in procedures like PCR, cloning, or any experiment involving RNA or DNA. -
Question:
How is DEPC-treated water prepared?
Answer: To prepare DEPC-treated water, diethylpyrocarbonate is added to distilled water and incubated to allow the DEPC to react with any RNases or DNases present. Following the treatment, the water is typically autoclaved to remove any residual DEPC and ensure sterility. This meticulous preparation process assures that the water achieves more than 99.99% purity, making it suitable for sensitive laboratory applications where contamination is a significant concern. -
Question:
What are the benefits of using lab-grade purified water in research?
Answer: Using lab-grade purified water provides numerous benefits, including enhanced accuracy and reliability of experimental results. It minimizes the risk of contamination, ensuring that your samples and reagents remain pure and unaffected by external variables. High-quality purified water is essential in molecular biology, biochemistry, and microbiology, as it helps to maintain the integrity of nucleic acids and proteins during experiments. -
Question:
Is DEPC-treated water suitable for cell culture applications?
Answer: DEPC-treated water is not typically recommended for cell culture applications as it contains compounds that may harm living cells. While it is effective for molecular biology experiments, it's better to use other types of sterile, DNase-free water specifically formulated for cell cultures to support cell growth and maintenance without introducing potential toxins present in DEPC-treated solutions. -
Question:
How do I store DEPC-treated water once it's prepared?
Answer: DEPC-treated water should be stored in sterilized, tightly-sealed containers to prevent contamination. It is best kept at 4°C to maintain its purity and effectiveness. Ensure that storage containers are made of materials compatible with the water’s chemical properties. Always label containers clearly, noting that they are DEPC-treated, to avoid any misuse in your laboratory. -
Question:
Can DEPC-treated water be used in routine laboratory procedures?
Answer: Yes, DEPC-treated water is widely used in various laboratory procedures, particularly in molecular biology techniques such as RNA extraction, RT-PCR, and sequencing. Its high level of purity and sterility ensures that it does not introduce contaminants that could compromise the results of these sensitive experiments. However, it should not be used in methods that require live cell applications. -
Question:
What makes DEPC-treated water different from regular purified water?
Answer: DEPC-treated water differs from regular purified water due to its specialized treatment with diethylpyrocarbonate, specifically designed to eliminate RNases and DNases. Regular purified water may still contain trace amounts of these enzymes, posing a risk in sensitive molecular biology experiments. Thus, DEPC-treated water is the preferred choice where the integrity of nucleic acids is paramount, ensuring optimal conditions for experimental success. -
Question:
Is DEPC-treated water sterile?
Answer: Yes, DEPC-treated water is typically autoclaved after the treatment process to ensure sterility. This diligent process eliminates microbial contamination, making it suitable for laboratory use where sterility is critical. When conducting experiments that require the utmost precision and cleanliness, using sterile DEPC-treated water safeguards the reliability of your results and provides confidence in your experimental setup. -
Question:
How can I confirm the purity of DEPC-treated water?
Answer: The purity of DEPC-treated water can be confirmed through various analytical methods, such as UV spectroscopy, which can detect contaminants based on their absorbance characteristics. For critical applications, it's also possible to run inclusion tests for RNase and DNase presence, ensuring that the water meets the stringent requirements for sensitive biological experiments. Utilizing high-quality sources and third-party certified products can further ensure purity levels. -
Question:
Where can I buy DEPC-treated water in Guernsey?
Answer: You can buy DEPC-treated water from Ubuy in Guernsey. Ubuy offers a variety of laboratory-grade purified water options, ensuring that you receive high-quality products for your research needs. With a focus on reliability and customer satisfaction, Ubuy provides a convenient shopping experience for professional-grade laboratory supplies.
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Pros
- Good quality materials
- Easy to use
- Stylish design
- High performance
- Reliable customer support
Cons
- Slightly heavier than expected
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GBP 21
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Features & Benefits
- Ultrapure water treated with DEPC for enhanced safety.
- Free from RNase, DNase, and proteinase for reliable research.
- Ideal for RNA-related experiments and various biochemical reactions.
- Stored properly, retains quality for up to 36 months.
- Ensures reliability and consistency in experimental results.
- Laboratory safety recommended during handling for optimal use.
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