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Item #:
44545681
Alcian Blue Solution, pH 2.5, 500ml
Item #:
44545681
GBP 96
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Alcian Blue Solution, pH 2.5 provides ready-to-use staining for sulfated and carboxylated mucopolysaccharides.
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What Stands Out
Optimal pH Level
The Alcian Blue Solution is precisely formulated at pH 2.5, ensuring maximum dye binding for accurate histological and cytological staining results in research and clinical applications.
High Purity
This solution boasts high purity, minimizing background interference during staining procedures, thus providing clearer and more reliable results in microscopy and tissue analysis.
Generous Volume
With a 500ml volume, this product offers excellent value for labs requiring extensive use, making it an economical choice for repeated staining protocols and long-term projects.
Product Details
- Sulfated Mucosubstances in Acidic Environments: Display as Blue
- Hyaluronic Acid: Stains Blue
- Sialomucins: Appear Blue
| Item Weight | 1.1 lbs (500 grams) |
Product Technical Details
| Department | All Ages |
| Manufacturer | Tyr Scientific LLC |
Who Should Buy?
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Histology Laboratories
Essential for staining glycosaminoglycans in tissue samples, enhancing visualization in histological examinations.
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Research Institutions
Ideal for researchers studying polysaccharides and carbohydrates in biological samples due to its reliable staining properties.
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Educational Settings
Useful in academic labs for teaching students about histological staining techniques and cellular structure analysis.
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General Use
Not suitable for casual or non-specialized staining needs outside of professional or laboratory settings.
Product Description
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Customer Questions & Answers
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Question:
What is Alcian Blue Solution used for?
Answer: Alcian Blue Solution is primarily used in histology and pathology for staining acidic mucins and glycoproteins in tissue samples. This solution helps in visualizing these specific components under a microscope, aiding in diagnosis and research. Researchers often utilize Alcian Blue in studies of connective tissues, tumor markers, and gastrointestinal disorders. Using this stain allows for a clear distinction between different types of mucins, which is vital in understanding various diseases. -
Question:
What is the significance of the pH level in Alcian Blue Solution?
Answer: The pH of Alcian Blue Solution at 2.5 is crucial as it enhances the solubility of the stain and its affinity for negatively charged mucopolysaccharides. This acidic environment is optimal for binding with sulfated and carboxylated groups present in mucins, resulting in more vivid staining. Proper pH ensures that users can achieve consistent and reliable results in their staining applications, making it a valuable tool in both diagnostic and research laboratories. -
Question:
Can Alcian Blue Solution be used on any type of tissue sample?
Answer: Alcian Blue Solution is particularly effective on various tissue types that contain mucins, such as gastrointestinal tissues, connective tissues, and some tumors. However, not all tissues will show the same level of staining. It is essential to choose the right fixatives and preparation methods to ensure optimal staining results. Many labs may conduct preliminary tests with different samples to ascertain the best conditions for visualization, thus enhancing the overall effectiveness of the staining protocol. -
Question:
How should Alcian Blue Solution be stored?
Answer: To maintain its effectiveness, Alcian Blue Solution should be stored in a cool, dark place, away from direct light and temperature fluctuations. Recommended storage conditions often include refrigeration to prolong the shelf life. Proper handling and storage are crucial, as exposure to light or heat can degrade the stain, leading to diminished staining quality. Laboratories typically label their storage bottles clearly and monitor conditions regularly to ensure optimal performance. -
Question:
Is Alcian Blue Solution safe to handle?
Answer: While Alcian Blue Solution is commonly used in laboratory settings, it is essential to handle it with care. Wearing appropriate personal protective equipment, such as gloves and goggles, is recommended to mitigate any risks associated with skin or eye contact. Users should also work within a fume hood if possible, as inhaling vapors may cause irritation. Education on proper safety measures can enhance user confidence while using the solution in research or clinical settings. -
Question:
How long does Alcian Blue staining take?
Answer: The staining duration with Alcian Blue Solution can vary based on the thickness of the tissue section and the specific protocol used. Generally, staining can take anywhere from 30 minutes to a few hours. Following specific experimentation protocols is essential to achieve optimal results, as factors like incubation time and washing intervals will directly affect the intensity of the stain. Laboratories often customize their protocols to suit their specific needs based on prior results. -
Question:
Can Alcian Blue Solution be mixed with other stains?
Answer: Yes, Alcian Blue Solution can be combined with other staining techniques to provide a comprehensive view of tissue morphology. For example, it can be used alongside Hematoxylin and Eosin (H&E) stains to visualize both cellular structures and mucin content in samples. However, proper protocols must be followed to ensure that stain interaction does not lead to inconsistencies or artifacts. Many pathologists employ combined staining to enhance the diagnostic value of tissue evaluations. -
Question:
What are some common applications of Alcian Blue in research?
Answer: In research, Alcian Blue Solution facilitates the examination of carbohydrate moieties in various biological tissues. It's widely used in studies related to developmental biology, cancer research, and gastrointestinal pathology. Moreover, it plays a pivotal role in investigations examining polysaccharide production in cells, contributing valuable insights into cell behavior and tissue response during pathological conditions. Its versatility makes it an integral part of histochemical research methodologies. -
Question:
Is Alcian Blue Solution suitable for frozen sections?
Answer: Yes, Alcian Blue Solution can be applied to frozen tissue sections, but it is imperative to ensure proper fixation and drying of the sections before staining. Adjustments in staining time and technique may be necessary as frozen sections can behave differently than formalin-fixed tissues. Many histotechnologists optimize their staining protocols based on their specific samples, ensuring that the frozen samples yield reliable and high-quality staining results. -
Question:
Where can I buy Alcian Blue Solution, pH 2.5, 500ml in Guernsey?
Answer: You can purchase Alcian Blue Solution, pH 2.5, 500ml from Ubuy in Guernsey. Ubuy offers a wide range of laboratory reagents and supplies, ensuring that you can access quality products for your research needs. They often provide detailed product descriptions and reviews, helping you make informed purchasing decisions.
Tyr Scientific LLC Analytical Reagents Editorial Review
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Pros
- High-quality staining agent
- Stable at low pH
- Versatile in applications
- Clear and consistent results
- 500ml size offers great value
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GBP 96
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Features & Benefits
- Ready-to-use reagent for histological visualization.
- Effectively stains sulfated and carboxylated mucopolysaccharides.
- Ideal for detecting glycoproteins like sialomucins.
- Produces consistent blue staining in acidic environments.
- Facilitates research and diagnostic applications.
- High-quality solution for reliable results.
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